Makhlouf Fatima Zahra | Université 8 Mai 1945 Guelma

Electrochemical sensors, Platinum-Nickel nanoparticles, Screen-printed electrodes, Paracetamol, 4-aminophenol, Ascorbic acid, Zinc, Simultaneous detection, Self-medication.

keywords (Ar)

Key words (Ar):

أجهزة استشعار كيميائية كهربائية، جزيئات نانو البلاتين-النيكل، أقطاب كهربائية مطبوعة بالشاشة، باراسيتامول، 4-أمينوفينول، حمض الأسكوربيك، زنك، الكشف المتزامن، الاستخدام الذاتي للأدوية.

keywords (Fr)

Key words (Fr):

Capteurs électrochimiques, nanoparticules de platine-nickel, électrodes sérigraphiées, paracétamol, 4-aminophénol, acide ascorbique, zinc, détection simultanée, automédication.

Self-medication with over-the-counter (OTC) analgesics like paracetamol (PA) and its toxic byproduct, 4-aminophenol (4-AP), is on the rise. While OTC analgesics are generally safe at recommended doses, overuse can lead to liver and kidney damage. Ascorbic acid (AA) and zinc (Zn(II)) are often combined with PA, but excessive intake can cause gastrointestinal, kidney, and neurological issues. The unmonitored use of these substances individually or in combination raises concerns about potential overdoses and adverse reactions, highlighting the need for reliable, sensitive detection methods to ensure patient safety. Due to the electroactive nature of PA, 4-AP, AA, and Zn(II), electrochemical sensors, particularly screen-printed electrodes (SPEs), have emerged as a promising tool. SPEs, typically consisting of a working electrode, counter electrode, and reference electrode on a single substrate, have gained widespread popularity in electroanalysis over the past few decades for offering advantageous material properties, including disposability, fast response times, compact size, low cost, and ease of portability.

This thesis presents the development of two electrochemical sensors based on screen-printed electrodes modified with Pt–Ni bimetallic nanoparticles for detecting paracetamol, 4-aminophenol, ascorbic acid, and zinc. The sensors, differing in their working electrodes (graphite vs graphene), utilize Pt–Ni nanoparticles for enhanced electro-catalytic performance, enabling both individual and simultaneous detection in pharmaceutical and biological samples.

Abstract (Ar)

Abstract (Ar):

إن استخدام الأدوية المسكنة التي تُصرف دون وصفة طبية مثل الباراسيتامول (PA) ومنتجه السام 4-أمينوفينول (4-AP)في تزايد. بينما تكون الأدوية المسكنة التي تُصرف دون وصفة طبية عادةً آمنة عند استخدامها بالجرعات الموصى بها، فإن الإفراط في استخدامها يمكن أن يؤدي إلى تلف الكبد والكلى. يتم دمج حمض الأسكوربيك (AA) والزنك (Zn(II)) غالبًا مع الباراسيتامول، ولكن الاستهلاك المفرط يمكن أن يسبب مشاكل في الجهاز الهضمي والكلى والجهاز العصبي. إن الاستخدام غير المراقب لهذه المواد سواء بشكل فردي أو معًا يثير القلق بشأن احتمال حدوث جرعات زائدة وردود فعل سلبية، مما يسلط الضوء على الحاجة إلى طرق كشف موثوقة وحساسة لضمان سلامة المرضى. نظرًا للطبيعة الكهروكيميائية للباراسيتامول و4-أمينوفينول وحمض الأسكوربيك والزنك، فقد ظهرت المستشعرات الكهروكيميائية، وخاصةً الأقطاب الكهربائية المطبوعة على الشاشة (SPE) ، كأداة واعدة. تعتبر الأقطاب الكهربائية المطبوعة على الشاشة، التي تتكون عادة من قطب عمل، وقطب ضد، وقطب مرجعي على ركيزة واحدة، قد اكتسبت شهرة واسعة في التحليل الكهروكيميائي في العقود الأخيرة بسبب خصائص المواد المميزة لها، بما في ذلك القابلية للتخلص منها، وأوقات الاستجابة السريعة، والحجم المدمج، والتكلفة المنخفضة، وسهولة حملها.

تستعرض هذه الأطروحة تطوير جهازين استشعار كيميائي كهربائي معتمدان على أقطاب كهربائية مطبوعة بالشاشة تم تعديلها بجزيئات نانو ثنائية المعدن من البلاتين-النيكل للكشف عن الباراسيتامول، 4-أمينوفينول، حمض الأسكوربيك، والزنك. تختلف المستشعرات في أقطابها العاملة (غرافيت مقابل جرافين)، وتستخدم جزيئات نانو البلاتين-النيكل لتحسين الأداء الكهرو-تحفيزي، مما يسمح بالكشف الفردي والمتزامن في العينات الصيدلانية والبيولوجية.

Abstract (Fr)

Abstract (Fr):

L'automédication avec des analgésiques en vente libre tels que le paracétamol (PA) et son sous-produit toxique, le 4-aminophénol (4-AP), est en augmentation. Bien que les analgésiques en vente libre soient généralement sûrs aux doses recommandées, une surconsommation peut entraîner des dommages au foie et aux reins. L'acide ascorbique (AA) et le zinc (Zn(II)) sont souvent associés au PA, mais une ingestion excessive peut causer des problèmes gastro-intestinaux, rénaux et neurologiques. L'utilisation non contrôlée de ces substances, individuellement ou en combinaison, soulève des inquiétudes concernant les risques de surdoses et de réactions indésirables, ce qui met en évidence la nécessité de méthodes de détection fiables et sensibles pour garantir la sécurité des patients. En raison de la nature électroactive du PA, du 4-AP, de l'AA et du Zn(II), les capteurs électrochimiques, en particulier les électrodes sérigraphiées (SPE), ont émergé comme un outil prometteur. Les SPE, qui se composent généralement d'une électrode de travail, d'une contre électrode et d'une électrode de référence sur un seul substrat, ont gagné une grande popularité dans le domaine de l'électroanalyse au cours des dernières décennies en raison de leurs propriétés matérielles avantageuses, telles que la jetabilité, des temps de réponse rapides, une taille compacte, un faible coût et une grande portabilité.

Cette thèse présente le développement de deux capteurs électrochimiques basés sur des électrodes sérigraphiées modifiées avec des nanoparticules bimétalliques Pt-Ni pour détecter le paracétamol, le 4-aminophénol, l'acide ascorbique et le zinc. Les capteurs, qui diffèrent par leurs électrodes de travail (graphite contre graphène), utilisent des nanoparticules de Pt-Ni pour améliorer les performances électro-catalytiques, ce qui permet une détection individuelle et simultanée dans des échantillons pharmaceutiques et biologiques.

Scientific publications

Scientific publications :

Scientific publications

Fatima Zahra Makhlouf, Mohamed Lyamine Chelaghmia, Rafiaa Kihal, Craig E. Banks, Hassina Fisli, Mouna Nacef, Abed Mohamed Affoune, Maxime Pontié, Screen-printed electrodes decorated with low content Pt–Ni microstructures for sensitive detection of Zn(II), ascorbic acid and paracetamol in pharmaceutical products and human blood samples, Microchemical J. 206 (2024) 111467. https://doi.org/10.1016/j.microc.2024.111467

Scientific conferences

Scientific conferences:

1- Fatima Zahra Makhlouf, Mohamed Lyamine Chelaghmia and Drissi Widad, “Platinum Nanoparticles Based Screen Printed Electrode for the Simultaneous Detection of Paracetamol and Ascorbic acid”. Oral presentation on physical mode in the first National Conference of Materials Sciences and Engineering, (MSE’22) held in Khenchla, Algeria, June 28 & 29th, 2022.

2- Fatima Zahra Makhlouf and Mohamed Lyamine Chelaghmia, “Electrochemical Sensing of Acetaminophen Using a Disposable Modified Screen Printed Electrode”. Poster on physical mode in the Second International seminar on Industrial Engineering and Applied Mathematics (ISIEAM’22) held in Skikda, Algeria, October 23 & 24th, 2022.

3- Fatima Zahra Makhlouf and Mohamed Lyamine Chelaghmia, “A highly Sensitive 4-aminophenol Sensor Based on a Platinum Nanoparticles Modified Screen-Printed Electrode”. Oral presentation on physical mode in the first International Conference on Materials Sciences and Technology (MatSience-2022) held in Khenchla, Algeria, December 13-15th, 2022.

4- Azzedine Makhlouf and Fatima Zahra Makhlouf, “Thermal behavior of isotactic polypropylene composite filled with microtalc (iPP/µTalc)”. Oral presentation on physical mode in the first International Conference on Materials Sciences and Technology (MatSience-2022) held in Khenchla, Algeria, December 13-15th, 2022.

5- Azzedine Makhlouf, Safieddine Bahlouli and Fatima Zahra Makhlouf, “Study of the Aging by RSM of a Biocomposite (Epoxy/Cork) in a Humid Environment”. Oral presentation on physical mode in the 1st International Seminar on Chemical Processes and Sustainable Development (ISCPSD'23) held in Skikda, Algeria, October 23-25th, 2023.

6- Fatima Zahra Makhlouf, Mohamed Lyamine Chelaghmia, Azzedine Makhlouf, Hassina Fisli and Abed Mohamed Affoune, “Electrochemical Sensing of Hydroquinone using a Nano-Modified Screen Printed Electrode for Quality Control of Tap Water”. Poster on physical mode in the 2nd International Conference on Electrochemical Sciences and Technology (ICEST’2024) held in Sétif, Algeria, May 7-9 th, 2024.

7- Fatima Zahra Makhlouf, Mohamed Lyamine Chelaghmia, Azzedine Makhlouf, and Maxime Pontié, “Nickel Nanoparticles-Decorated Graphite-Modified Screen Printed Electrode toward the Electrochemical Determination of Dopamine”. Oral presentation on online mode in the 3rd Edition of the International Conference on Materials Science and Engineering and their Impact on The Environment (ICMSE’2024) held in Sidi bel abbes, Algeria, May 29 & 30 th, 2024.

8- Fatima Zahra Makhlouf, Mohamed Lyamine Chelaghmia and Azzedine Makhlouf, “Comparative Study of Metrohm 110 Screen Printed Electrode Modifications: Nickel Nanoparticles versus Nickel Tetrasulfonated Phthalocyanine for Enhanced 4-Aminophenol Detection: Elaboration and Characterization”. Oral presentation on online mode in the National Seminar on Nanomaterials: Synthesis and Applications held in Boumerdes, Algeria, June 5 & 6 th, 2024.

9- Fatima Zahra Makhlouf, Mohamed Lyamine Chelaghmia and Azzedine Makhlouf, “Screen-Printed Electrode Modified with Pt-Nanoparticles as an Advanced Material for Simultaneous Quantification of Ascorbic Acid and Dopamine”. Oral presentation on physical mode in the Premier Séminaire National Hybride de Chimie (SNHC’24) held in Batna, Algeria, October 26 & 27 th, 2024.

10- Azzedine Makhlouf and Fatima Zahra Makhlouf, “Aging study of new composite”. Oral presentation on physical mode in the Premier Séminaire National Hybride de Chimie (SNHC’24) held in Batna, Algeria, October 26 & 27 th, 2024.

11- Fatima Zahra Makhlouf, Mohamed Lyamine Chelaghmia, Abed Mohamed Affoune and Nahla Kadri, “Pt-Ni Nanoparticles-Based Screen-Printed Electrode for the Electrochemical Detection of p-Acetylaminophenol and Its Toxic Impurity p-Aminophenol”. Oral presentation on physical mode in the 3ème Séminaire National sur la Chimie Appliquée (SNCA’3) held in Guelma, Algeria, November 14 th, 2024.

12- Nahla Kadri, Mohamed Lyamine Chelaghmia, Ghediri Dalia, Fatima Zahra Makhlouf, Hassina Fisli and Abed Mohamed Affoune, “Electrochemical Determination of Trace of Mercury Ions using Functionalized Screen-Printed Electrode”. Oral presentation on physical mode in the 3ème Séminaire National sur la Chimie Appliquée (SNCA’3) held in Guelma, Algeria, November 14 th, 2024.

13- Fatima Zahra Makhlouf, Mohamed Lyamine Chelaghmia, Abed Mohamed Affoune and Azzedine Makhlouf, “Development of Screen-Printed Graphite Electrodes Modified with Platinum and Nickel Nanoparticles for the Simultaneous Quantification of Vitamin C and Acetaminophen in Pharmaceutical Samples”. Oral presentation on physical mode in the International Conference on Material and Mechanic (ICMM'2024) held in Boumerdes, Algeria, November 20 & 21 th, 2024.

14- Fatima Zahra Makhlouf, Mohamed Lyamine Chelaghmia, Rafiaa Kihal, Azzedine Makhlouf and Nahla Kadri, “A Novel Disposable Electrochemical Sensor for Simultaneous Triple Detection of Paracetamol, Ascorbic Acid, and Zinc in Real-World Samples ”. Oral presentation on physical mode in the 7th International Symposium on Chemistry (CIC-7) held in Batna, Algeria, November 27- 29 th, 2024.